Science is a core subject in the curriculum in which all students experience ways of understanding the natural world. The aim of the Science Department is to ensure that students leave Pembroke School with a good scientific understanding for decision-making and an awareness of the scientific issues facing our society.

A multidisciplinary Science staff with a diverse range of experiences and interests offers 'hands on' science programmes incorporating experimental design and problem solving skills. Enrichment of the programme occurs by students being involved in activities at places like CSIROSec (the educational section of the Commonwealth Scientific and Industrial Research Organisation), Monarto Zoo, Old Watulunga (Pembroke's Outdoor Education property), the Adelaide Zoo, the Botanic Gardens and the South Australian Museum.

The flexibility of the Science programme through its SACE and IBD options allows students' individual needs to be accommodated. In addition, students are also given the opportunity to expand their horizons by participation in the Oliphant Science Awards, The Australian National Chemistry Quiz, International Competitions and Assessments for Schools: Science (ICAS Science) and the Australian Science Olympiad Programme.